Polymerase chain Reaction

Title: Polymerase chain Reaction
Category: Science & Technology
Details: Words: 562 | Pages: 2.4 (approximately 235 words/page)

Polymerase chain Reaction

Introduction to PCR lab In this experiment polymerase chain reaction (PCR) is used to amplify a short piece of DNA from the human chromosome 8. A short insertion of a DNA sequence, in this case Alu, which is found to be within the “tissue plasminogen activator gene on chromosome 8, is observed very carefully. Even though the DNA from human to human does not differ a whole lot and has many similarities the diversity among regions of …showed first 75 words of 562 total…

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…showed last 75 words of 562 total…an unamplified control of PCR. After Electrophoresis and staining the products will be visible as dark bands on the gel. By measuring how far each of these liquids traveled through the gel we can examine whether the person has the TPA-25 insertion. If two bands show up the person is said to be heterozygous meaning one allele has it the other doesn’t. The control just gives us assurance that the PCR was not contaminated.

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